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抗沙门氏菌单克隆抗体(捕获)

抗沙门氏菌单克隆抗体(捕获)

产品编号

ANAB1002

别名

Salmonella

规格
  • 1mg
产品介绍
Catalog NumberANAB1002
AliasSalmonella
Size1mg
Product Description抗沙门氏菌单克隆抗体(捕获)(ANAB1002)由小鼠杂交瘤细胞上清中亲和纯化获得,免疫原是沙门氏菌抗原。
Molecular NameSalmonella
Product IntroductionSalmonella:沙门氏菌,是一类重要的食源性人畜共患病原菌,可导致肠胃炎、败血症等疾病。
Species沙门氏菌抗原
HostMouse
ClonityMonoclonal
Purity> 85% as determined by Tris-Bis PAGE
PurificationProtein A/G
Storage ConditionSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
FormulationPBS with 0.05% proclin300,50% glycerol,pH7.3.
Shipping ConditionIn general, liquid samples are shipped on dry ice and lyophilised samples are shipped on blue ice.
BackgroundRelated high molecular weight plasmids of several serotypes and species of Salmonella have been associated with virulence in a variety of animal models of infection. The primary virulence plasmid phenotype is in the ability of salmonellae to spread beyond the initial site of infection, the intestines.
Immunogen沙门氏菌抗原
ApplicationSDS-PAGE, ELISA
Product Declaration该产品仅供科研使用,不可直接用于人体或注射。
FAQ
  • 什么是“捕获”抗体和“检测”抗体?它们在ELISA实验中是如何配合工作的?

    这是在夹心法ELISA(特别是双抗体夹心法)中使用的两种抗体。(1)捕获抗体: 通常被预先包被在ELISA板子上,负责“捕获”或“结合”样品中的目标抗原。(2)检测抗体:在抗原被捕获后加入,负责与抗原的另一个位点结合,并通过其携带的标记物(如酶HRP)来产生信号。(3)工作流程:样品中的抗原先被“捕获抗体”抓住,然后“检测抗体”与之结合,形成“捕获抗体-抗原-检测抗体”的“三明治”复合物。最后通过加入底物,由检测抗体上的酶催化产生颜色变化,从而进行定量或定性分析。

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