HCoV-229E Spike Protein(ECD), His Tag_天津卡梅德科技发展有限公司

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HCoV-229E Spike Protein(ECD), His Tag

HCoV-229E Spike Protein(ECD), His Tag

产品编号

KMP4826

别名

人类冠状病毒刺突蛋白, HCoV-229E Spike, S Protein, HCoV-229E Spike Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类冠状病毒刺突蛋白, HCoV-229E Spike, S Protein, HCoV-229E Spike Protein
Catalog Number	KMP4826
Product Description	The HCoV-229E (HCoV-229E) Spike Protein(KMP4826) is produced in Insect Cells. A DNA sequence encoding the human coronavirus (HCoV-229E) spike protein (S1+S2 ECD) (APT69883.1) (Cys16-Trp1115) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Molecular Name	HCoV-229E Spike
Species	HCoV-229E
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-229E Spike蛋白的宿主受体是什么？

结合宿主细胞表面的氨基肽酶N（APN），介导病毒入侵。
Q2-该蛋白能否用于通用冠状病毒疫苗研究？

是的，结合其他β冠状病毒Spike蛋白，可探索交叉保护性表位。
Q3-如何验证其受体结合活性？

通过表面等离子共振（SPR）检测与重组APN蛋白的亲和力（KD值）。
Q4-是否提供糖基化修饰数据？

可提供质谱分析报告，确认N-糖基化位点（如Asn-370）。
Q5-运输条件有何要求？

干冰运输，到货后立即-80℃保存，避免反复冻融。

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